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KMID : 0368420110540010057
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Journal of Plant Biology
2011 Volume.54 No. 1 p.57 ~ p.64
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Molecular Identification and Physiological Characterization of a Putative Novel Plasma Membrane Protein from Arabidopsis Involved in Glucose Response
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Chung Moon-Soo
Huang Ping
Ha Chan-Man
Jun Ji-Hyung
Ahn Sung-Ju
Zhang Fu-Chun
Bae Hyun-Jong
Cho Baik-Ho
Kim Cheol-Soo
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Abstract
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We carried out functional analysis of a putative novel Arabidopsis plasma membrane glucose-responsive regulator, designated AtPGR, which contains seven predicted transmembrane domains. Several evidences showed that AtPGR is a glucose-related protein, but its biological functions have yet to be reported in any plant. Analyses of the AtPGR promoter-β-glucuronidase (GUS) construct and RNA in situ hybridization revealed substantial gene expression in the vasculature of various tissues, especially in the phloem region. Glucose treatment induced the highest levels of GUS activity, reaching a peak at 3 h and declining thereafter, consistent with glucose-mediated regulation of the AtPGR promoter. We generated an atpgr RNAi knockdown mutant and found that this plant grew and developed normally. Ectopic expression of AtPGR gene modulated the induction of glucose and 2-deoxyglucose insensitivity under stress conditions. By way of contrast, cotyledon greening of atpgr RNAi knockdown mutant seeds enhanced sensitivity to glucose and 2-deoxyglucose. Taken together, these results suggest that AtPGR functions as a potential glucose-responsive regulator in carbohydrate metabolism.
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KEYWORD
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At5g19930, Glucose, Glucose insensitivity, Glucose regulator, Phloem, Plasma membrane
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